General Cell Based Assays
Molecular Devices Fura-2 QBT Calcium Kit, Bulk, Ratiometric, 10 Vials
The Fura-2 QBT Calcium Kit (bulk) is a homogeneous, mix-and-read ratiometric assay that uses Fura-2 dye with extracellular masking technology to reduce wash steps and minimize assay variability. The bulk format supplies multiple vials for high-throughput screening on 96- and 384-well microplates.
- Contains 10 vials of Component A
- Each vial sufficient for approximately 10 microplates
- Total kit coverage approximately 100 microplates (96- or 384-well)
- Assay uses ratiometric Fura-2 dye, dual-excitation (340/380 nm), emission 510 nm
- Compatible with fluorescence plate readers and FLIPR/FLIPR Tetra systems
- Assay buffer (Component B) not included and must be obtained separately
- Free of animal-derived components
Cell Biolabs Inc OxiSelect™ Comet Assay Kit (3-Well Slides), 75 assays (25 slides)
The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic “comet tail” shape under the microscope. The extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters. The OxiSelect™ Comet Assay is a fast and sensitive kit for the measurement of cellular DNA damage. Each kit provides sufficient reagents to perform up to 25 assays (25 slides with 3 wells each).
Eurofins Discoverx PathHunter Human GPR132 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR132 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR132 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Enzo Life Sciences Matrix Metalloproteinase-13 (MMP-13) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-13, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet. Alternative name: Collagenase 3. Applications: Fluorescent detection, HTS Activity assay.
Eurofins Discoverx PathHunter Human NF-kB (RELA)-IkB Nuclear Translocation Cell-Based Assay Kit (HEK 293)
The PathHunter Human NF-kB (RELA)-IkB Stable Cell Line Nuclear Translocation Assay (HEK 293) contains a stable clonal cell line used to measure NFκB pathway activation via nuclear translocation of RELA. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Electron Microscopy Sciences Flim Sleeve 135 mm/20 exp with Frosted Open Flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Frosted Open Flap
Eurofins Discoverx PathHunter Human NPFFR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human NPFFR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure NPFFR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress MRGPRX2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0309E2CP0M)
The PathHunter MRGPRX2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cortistatin-14) based activation of MRGPRX2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX2 and EA-tagged β-Arrestin-2. Activation of MRGPRX2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human FSHR β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human FSHR β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure FSHR (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human DRD2S Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human DRD2S Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD2S (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Amsbio LLC Cupric Iron Chelating (CIC) Assay Kit -store kit components as indicated
Cupric Iron Chelating (CIC) Assay Kit -store kit components as indicated
Eurofins Discoverx PathHunter CXCR5 Bioassay Kit, 10-Plate. (93-0514Y2-00064)
The PathHunter CXCR5 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CXCL13) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR5 and EA-tagged β-Arrestin-2. Activation of CXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter Human CD33 (SIGLEC3) Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human CD33 (SIGLEC3) Stable Cell Line Internalization Assay (U2OS) measures internalization of the CD33 receptor via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Apicidin, HDAC inhibitor, 1MG
MW 623.8 Da, Purity >95%. Potent histone deacetylase inhibitor (IC₅₀ = 0.7 nM). Inhibits cancer cell proliferation via induction of p21WAF1/Cip1 and gelsolin. Active in vitro and in vivo. Cell-permeable.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical MItoLIteRed FX600 500test
A mitochondrial dye for live and fixed cells; ex/em = 575/600 nm