General Cell Based Assays
Eurofins Discoverx PathHunter eXpress F2RL3 (PAR4) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0279E2CP0L)
The PathHunter F2RL3 (PAR4) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serine proteases) based activation of F2RL3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL3 and EA-tagged β-Arrestin-2. Activation of F2RL3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress LGR5 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0526E2ACP1L)
The PathHunter LGR5 (GPR49) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. R-spondins) based activation of LGR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR5 and EA-tagged β-Arrestin-2. Activation of LGR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Adalimumab Bioassay Kit, 2-Plate. (93-0538B15-00131)
The PathHunter Adalimumab Bioassay Kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, cell plating reagent, control agonist, detection reagents, and assay plates. The ready-to-use cryopreserved cells have been manufactured for single-use to ensure assay reproducibility, and faster implementation from characterization to lot release. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter TrkB-P75 Bioassay Kit, 2-Plate. (93-0530Y3-00075)
The PathHunter TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Upon ligand-induced dimerization of TrkB-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx AssayComplete Cell Plating 20 Reagent, 100. (93-0563R20A)
AssayComplete Cell Plating 20 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress LGR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0527E2ACP1M)
The PathHunter LGR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR6 and EA-tagged β-Arrestin-2. Activation of LGR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress LGR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0527E2ACP1L)
The PathHunter LGR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR6 and EA-tagged β-Arrestin-2. Activation of LGR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TrkB-P75 Functional Assay, 2-Plate Kit. (93-0530E3CP16M)
The PathHunter eXpress TrkB-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Exendin-4 Bioassay Kit, 10-Plate. (93-0300Y2-00030)
The PathHunter Exendin-4 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exendin-4 based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR109A CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0301E2CP2L)
The PathHunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109A and EA-tagged β-Arrestin-2. Activation of GPR109A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER4 HEK 293 β-Arrestin GPCR Assay, 10pl Kit. (93-0304E1CP0L)
The PathHunter PTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER4 and EA-tagged β-Arrestin-2. Activation of PTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2A U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0401E3CP19L)
The PathHunter HTR2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2A and EA-tagged β-Arrestin-2. Activation of HTR2A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRA1B CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0274E2CP0L)
The PathHunter ADRA1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA1B and EA-tagged β-Arrestin-2. Activation of ADRA1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP2 (AM1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0270E2CP0L)
The PathHunter CALCRL-RAMP2 (AM1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP2. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PPYR1 (NPY4R) CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0233E2CP0M)
The PathHunter PPYR1 (NPY4R) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. pancreatic polypeptide) based activation of PPYR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PPYR1 and EA-tagged β-Arrestin-2. Activation of PPYR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.