General Cell Based Assays
FUJIFILM BIOSCIENCES INC Phos-tag Mass Analyl Kit 1set
Phos-tag Mass Analytical Kit is mixed with samples for MALDI-TOF/Mass analysis Phosphorylated molecule-Phos-tag complex is detected in a positive mode and phosphorylated molecules usually difficult to detect can be detected with improved sensitivity Three types of reagents contain different types of zinc
Innovative Research Inc Rat Act tPA Kit
Rat Active tPA ELISA Kit - This rat tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator (tPA) in rat plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Functionally active rat tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active tPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Dojindo Molecular Technologies Inc MDA(malondialdehyde) Assay Kit, 100test, Capable of measuring MDA in cells and tissues, Simple procedure.
Malondialdehyde (MDA) is one of the end products of lipid peroxides, the MDA concentration in cell or tissue samples can indicate lipid peroxidation which is important in the fields of oxidative stress and ferroptosis. MDA is also called reactive aldehyde and reacts with amino and thiol groups to cause protein denaturation and DNA damage, so it is also measured in various disease studies such as cancer and diabetes. Dojindo's MDA Assay Kit uses the TBARS method to detect MDA by measuring the fluorescence or absorbance of the adduct of MDA and thiobarbituric acid that develops color according to the amount of MDA.
Medchemexpress LLC SDMA (Synonyms: Symmetric dimethylarginine; NG,NG'-Dimethyl-L-arginine) 5mg
SDMA (Symmetric dimethylarginine) is an endogenous inhibitor of nitric oxide (NO) synthase activity. SDMA, a novel kidney biomarker, permits earlier diagnosis of kidney disease than traditional creatinine testing.
Eurofins Discoverx PathHunter eXpress GPR78 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0370E2ACP1M)
The PathHunter GPR78 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR78 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR78 and EA-tagged β-Arrestin-2. Activation of GPR78 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NADNADH Cel-Based asy KIt 1ea
Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric ethod for measuring intracellular NAD+and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.
Revvity Health Sciences Inc HTRF Human Phospho-VEGF receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
HTRF Human Phospho-VEGF receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points