General Cell Based Assays
Eurofins Discoverx PathHunter Human APRIL (BCMA) Signaling Reporter Cell-Based Assay Kit (K562)
The PathHunter Human APRIL (BCMA) Signaling NF-kB Reporter Stable Cell Line Assay (K562) monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC). This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Iron Assay Kit (Colorimetric)
Iron Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Glutathione Assay Kit, 480 Well
Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative protocol. The GSH Assay can be used for plasma, tissue samples, and cultured cells with minimal sample processing.
Eurofins Discoverx PathHunter eXpress DRD2L (Long Isoform) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0579E2CP2L)
The PathHunter DRD2L eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2L GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD2L and EA-tagged β-Arrestin-2. Activation of DRD2L stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter AGTR1 Bioassay Kit, 2-Plate. (93-0312Y2-00125)
The PathHunter AGTR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTR1 and EA-tagged β-Arrestin-2. Activation of AGTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical DUB Activity Assay Kit, 96 Well
Cayman’s DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in a 96-well plate format for multi-sample analysis. Each kit contains sufficient material for one full 96-well plate assay set-up to be run. Ubiquitin-AMC is not a suitable substrate for all DUBs. Compatibility must be determined by the end user.
Eurofins Discoverx PathHunter eXpress mGLP1R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0478E2MCP0M)
The PathHunter mGLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-2. Activation of mGLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC QuickZyme Hydroxyproline Assay Kit 5 X 96-Assays (for collagen analysis)
QuickZyme Hydroxyproline Assay Kit, 5 x 96-Assays. Quantitative hydroxyproline measurement for collagen analysis. The ONLY hydroxyproline-based assay that does NOT require a drying step following acid hydrolysis of collagen, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Broad application: conditioned media, cells/cell extracts, tissues/tissue extracts. Species & collagen type independent. Dynamic range: 6-300 uM. Sensitivity: 2.4 uM. Sample vol. 5-35 ul. Measures total hydroxyproline present in the sample. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all types of collagen present without discriminating between the types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.
Eurofins Discoverx PathHunter eXpress P2RY4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0263E2CP0M)
The PathHunter P2RY4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY4 and EA-tagged β-Arrestin-2. Activation of P2RY4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc DAG (Diacylglyercol) Assay Kit, 100 assays
Diacylglycerols (DAG) are key intermediates in the biosynthesis of many cellular lipids and play a fundamental role in biochemical signaling. Cell Biolabs’ DAG Assay Kit simple 96-well fluorometric assay that measures diacylglycerol content in samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG samples, yielding phosphatidic acid. Next, a lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Finally, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit’s fluorometric probe. The kit contains a DAG Standard and has a detection sensitivity limit of 15 µM. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. Note each sample replicate requires 2 assays, one of which is a negative control to be subtracted as background.
Indigo Biosciences Human Macrophage Colony-Stimulating Factor Receptor (M-CSFR) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Macrophage Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against M-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Abcam PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase)
PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase)
The product is subject to the following: Abcam Restricted Use Statement
Genscript Corporation DPPIV/CD26 His Human
CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body and a soluble form is present in body fluids There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent